Title: Laboratory Security in the Use of Hazardous Biological and Chemical Agents
Policy Number: 1.8.12
Date: 12/05/2001


The mission of the College of Veterinary Medicine (CVM) includes instructing veterinary and graduate students in the pathogenesis, diagnosis, and treatment of animal diseases, including those caused by intoxicants and zoonotic infectious agents. The College mission also includes research into biological and chemical agents involved in the etiology of these diseases. Both the CVM and the Oklahoma Animal Disease Diagnostic Laboratory are charged with protecting the animal resources of the State of Oklahoma and assisting the State Department of Health in the control and prevention of diseases caused by intoxicants and zoonotic infectious agents. These missions are consistent with the expectation that the veterinary medical profession serve the interests of public health and must be performed in strict accordance with federal and state regulations promulgated by the National Institutes of Health (NIH) and the Centers for Disease Control (CDC), with appropriate attention to security of hazardous materials.


1) As used in this policy, the term “hazardous biological or chemical agents” refers to agents included on the CDC Select Agent Transfer list, infectious agents subject to Biosafety Level-3 containment, and any biological or chemical agents deemed hazardous by the Research Advisory Committee (listing attached). The latter shall include, but not be restricted to, shigatoxin-producing strains of bacteria, antibiotic-resistant zoonotic bacteria, and chemicals used as biowarfare agents. Determination of required Biosafety Level containment will be based on published guidelines from the NIH, Guidelines for Research Involving Recombinant DNA Molecules.

2) Whenever hazardous biological or chemical agents are in use, the outer laboratory door shall be locked and a “NO ENTRY” sign shall be posted.

3) Hazardous biological and chemical agents will be stored in an appropriate locked container (e.g., freezer, refrigerator, cabinet). Due diligence shall be exercised to secure the container against unauthorized access.

4) All personnel involved in the use of a hazardous biological or chemical agent, including those working in the laboratory in which the agent is stored and used, shall receive training relevant to the hazard posed by that agent.

5) An inventory documenting amounts, storage conditions, and use of hazardous biological and chemical agents, and training records shall be maintained in the laboratory and shall be subject to quarterly inspection by the Laboratory Safety Officer for the College of Veterinary Medicine.
Appendix A to Part 72 – Select Agents

13 Viruses
Crimean-Congo haemorrhagic fever virus
Eastern equine encephalitis virus
Ebola viruses
Equine morbillivirus
Lassa fever virus
Marburg virus
Rift Valley Fever virus
South American haemorrhagic fever viruses
Tick-borne encephalitic complex viruses
Variola major virus
Venezuelan Equine Encephalitis virus
Viruses causing hantavirus pulmonary syndrome
Yellow fever virus

9 bacteria
Bacillus anthracis
Brucella abortus, mellitensis, suis
Burkholderia mallei
Burkholderia pseudomallei
Clostridium botulinum
Francisella tularensis
Yersinia pestis

3 Rickettsiae
Coxiella burnettii
Rickettsia prowazekii
Rickettsia rickettsii

1 Fungi
Cocciodides immitis

12 Toxins
Botulinum toxins
Clostridium perfringens epsilon toxin
Staphylococcal enterotoxins
T-2 toxin

NIH Guidelines - Appendix B-III
Risk Group 3 Agents

14 Viruses/Prions
Alphaviruses – Group A Arboviruses
Semliki Forest virus
St. Louis encephalitis virus
Venezuelan Equine Encephalitis virus

Lymphocytic choriomeningitis virus

Hantaviruses including Hantaan virus
Rift Valley Fever virus

Flaviviruses– Group B Arboviruses
Japanese encephalitis virus
Yellow fever virus

Monkeypox virus

Transmissible spongioform encephalopathies agents

Human immunodeficiency virus types 1 and 2
Human T-cell lymphotropic virus types 1 and 2
Simian immunodeficiency virus

Vesicular stomatitis virus

10 bacteria
Brucella abortus,canis, mellitensis, suis
Burkholderia mallei
Burkholderia pseudomallei
Francisella tularensis
Pasteurella multocida type B
Yersinia pestis

10 Rickettsiae
Coxiella burnettii
Rickettsia akari, australis, Canada, conorii, prowazekii, rickettsii, siberica, tsutsugamushi, typhi

2 Fungi
Cocciodides immitis
Histoplasma capsulatum

Others as listed in the Footnotes and References of Sections I through IV – NIH Guidelines

This policy was introduced by Dr. Jerry R. Malayer, Associate Dean for Research & Graduate Education and was approved at the December 5, 2001, VAC meeting.

Approved By: Michael D. Lorenz
Reviewed By: VAC
Review Date: 12/05/2001